NGS.png

Next-Gen Sequencing

A Precision Diagnostic Tool

To Identify Dominant Infection-Causing Microbial Species. 

Next-generation sequencing (NGS) is transforming the field of genomics. Synergy Laboratories is at the forefront with leading researchers. Next-generation sequencing generates a massive amount of DNA data from which hundreds of microorganisms can be identified.

 

It can also reveal antibiotic resistance, the key to accurate treatment.

AdobeStock_246181676.jpeg

The Future of Testing Now 

Our High Complexity Genetics Laboratory extracts the DNA of all the microbes within a samples and matches those sequence codes to all 25,000+ known microbial species with extremely high accuracy and sensitivity.

NGS analyzes the whole patient sample and identifies all of the pathogens present that PCR and culture-based tests can miss.​ NGS does not require live organisms, special media, or growth conditions. Plus, Synergy offers rapid turnaround time for molecular testing -- most instances within 48 hours.

What's causing my patient's infection?

Infections are caused by pathogens - microorganisms that invade the body. These include bacteria, fungi, and parasites. Pathogens have unique genetic signatures that, in addition to determining their traits, can be used to identify them. Next-Generation Sequencing (NGS) technology can be used to identify which pathogens are making a patient sick. 

samples-infographic.png

More accurate diagnosis.
Better treatment outcomes.

The use of clinical criteria alone (culture and sensitivity) has been shown to have an error rate of up to 33%. Additionally, positive culture results do not always indicate a diagnosis of infection. Studies have shown that NGS has allowed for more accurate diagnosis and better treatment outcomes than traditional culture and sensitivity.1

While culture can inexpensively detect aerobic species in a short time span of 12-48 hours, difficult species, growth rates, and organisms in low abundance can easily be missed. Furthermore, anaerobic organisms, which are usually present in polymicrobial infections, are not usually identified in routine cultures. This can delay optimal treatment, especially in patients with complex or chronic infections.

Antibiotic susceptibility testing also has limits in traditional culture as antibiotic resistance genes present in one or more organisms can be horizontally transferred to other organisms. This can limit the successful treatment of the infection if any other species or resistance genes are present.2

 

Next-generation sequencing thus takes the guess work out of the diagnosis and subsequent treatment of infections by providing a complete 360° picture for the clinician.

1McDonald, M, et al., 2017, ‘A Head-to-Head Comparative Phase II Study of Standard Urine Culture and Sensitivity Versus DNA Next-generation Sequencing Testing for Urinary Tract Infections’, Reviews in Urology, vol. 19, no. 4, pp. 213-220. 2Alexander, Rachel, “The Need for NGS Technology in Infections Disease Diagnostics”, ASCLS Today, vol. 32, no. 4, 2018.